Effect of gold nanorod surface chemistry on cellular response.

نویسندگان

  • Christin Grabinski
  • Nicole Schaeublin
  • Andy Wijaya
  • Helen D'Couto
  • Salmaan H Baxamusa
  • Kimberly Hamad-Schifferli
  • Saber M Hussain
چکیده

Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG(5000)) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively low uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference in gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure.

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عنوان ژورنال:
  • ACS nano

دوره 5 4  شماره 

صفحات  -

تاریخ انتشار 2011